THE SMART TRICK OF GENETIC METHYLATION TESTING THAT NOBODY IS DISCUSSING

Treatment with sodium bisulfite deaminates unmethylated cytosine to uracil, While 5mC will not be influenced. Subsequent PCR amplification will switch the uracils by thymines. Reads from NGS libraries designed in parallel with and without bisulfite treatment are mapped onto reference genomes and the contrasting C/T positions involving the libraries